The dynamic Nexus: Gap junctions control protein localization and mobility in distinct and surprising ways Sean McCutcheon, et al.,?bioRxiv - Cell Biology 2020 Quote:?... For standard FRAP experiments N2A and HeLa cells were plated into 8-well imaging chambers (iBidi, cat no. 80826 or In Vitro Scientific,?C8-1.5H-N) and each well was transfected with 0.5 μg of each plasmid to drive expression of Connexin-fluorescent protein fusions 24-72 h prior to imaging ...