RFect小核酸轉染試劑/siRNA轉染試劑

RFect siRNA/miRNA Transfection Reagent

RFect小核酸轉染試劑/siRNA轉染試劑

產品簡介

RFect小核酸轉染試劑是國際知名科學家崔坤元博士領導我公司研發團隊在美國西雅圖實驗室研發成功的一種新型的小核酸轉染試劑。RFect可用來轉染siRNA、miRNA mimics、miRNA inhibitor 等200bp以內的小分子RNA，轉染細胞包括絕大多數貼壁生長的細胞，如一般細胞株、腫瘤細胞株等。目前，無論國外還是國內，轉染試劑的主要成分均為脂質體或聚乙烯亞胺(Polyethylenimine, PEI)，這兩種成分都具有很大的細胞毒性，并且轉染效果不好。RFect采用新型的動物源性的納米材料，毒性很低并且擁有非常卓越的轉染性能。與其它品牌的小核酸轉染試劑相比，RFect的細胞轉染陽性率一般在90%以上，Lamin A/C 基因抑制效率在95%以上，而其它品牌轉染試劑的細胞轉染陽性率一般不超過70%，Lamin A/C 基因抑制效率不超過75%。RFect細胞毒性很低，轉染細胞死亡率不到10%，而其它品牌試劑的轉染細胞死亡率一般在30%以上。血清對轉染效果沒有影響，不必刻意添加或更換培養液。本產品適用于細胞株轉染，原代細胞轉染，請選擇RFectPM小核酸轉染試劑。

產品特點

·?卓越的細胞轉染性能：細胞轉染陽性率高達90%以上，基因敲除效果明顯，A549細胞Lamin A/C 基因敲除效率在95%以上；

·?極低的細胞毒性：轉染細胞死亡率不到10%，大大降低了因細胞毒性對實驗結果的影響；

·?轉染細胞范圍廣，絕大多數貼壁細胞株都能獲得比較理想的轉染結果。


Fig. 1. RFect sRNA transfection reagent offers the highest transfection efficiency. ?HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using RFect sRNA?transfection reagent. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A The fluorescence images show the fluorescence of labeled siRNA.B.The fluorescence images show the fluorescence of labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).

Fig..2.?RFect siRNA?transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFect??In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

Fig..3.RFect?siRNA?transfection reagent offers high level of gene knockdown with low toxicity.?A549 cell lines were transfected with Lamin A/C siRNA (final concentration: 10nM) using RFect?siRNA transfection reagent.?RFect concentrations added to the cells ?exceeding transfection concentration of?20 times, the cells still do not produce significant toxicity, which makes these product?suitable?to optimization.?Lamin A/C knockdown was measured by qRT-PCR.

Fig.4.RFect siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using RFect.
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適用細胞

293T（人腎上皮細胞），A549（人肺癌細胞），HEK 293（人胚腎細胞），HeLa（人宮頸癌細胞），MCF-7（人乳腺癌細胞），HepG2（人肝腫瘤細胞），HT-1080(人纖維肉瘤），HLF-a（人肺細胞），Huh-7（人肝癌細胞），KB（人口腔表皮樣癌細胞），PC3（人前列腺癌細胞），BRL-3A（大鼠正常肝細胞），DU 145（人前列腺癌細胞），Hepa 1-6（小鼠肝癌細胞），OVCAR3（人卵巢癌細胞），NCaP-FGC（人前列腺癌細胞），C2C12（成肌細胞），MDCK（犬腎細胞），CHO-K1，islk.219細胞（人肺癌細胞），HT-29（人結腸癌細胞），NCI-H1688（人典型小細胞肺腫瘤/癌細胞），RKO結腸癌細胞，Vero（非洲綠猴腎細胞），COS-1（非洲綠猴腎成纖維細胞），COS7-L（非洲綠猴腎成纖維細胞），COS-7（非洲綠猴腎成纖維細胞），SK-BR3（人乳腺癌細胞），Hepa 1cLc7（鼠肝癌細胞），Daoy（人髓母細胞瘤細胞），DBTRG-05MG（人腦膠質母細胞瘤細胞），KLN 205（鼠肺磷癌細胞），Neuro-2a（小鼠神經瘤母細胞），DI-TNC1（大鼠腦間質細胞）等。

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